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keyence_sop [2020/11/12 18:06] – furstkeyence_sop [2021/04/27 17:41] (current) – furst
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 Group or Company Group or Company
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 ====Overview:==== ====Overview:====
   * This training provides an introduction to using and operating the Keyence microscope including:   * This training provides an introduction to using and operating the Keyence microscope including:
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     - Follow steps to initialize the XY stage     - Follow steps to initialize the XY stage
   - **Tilting the head**   - **Tilting the head**
-    - The microscope head is able to tilt up to 90 degrees clockwise in order to better image samples. Caution should be taken to avoid crashing the objective into the sample or stage when head is tilted. \\ {{:tools:keyencemicroscope:lockout.jpg?400|}}+    - The microscope head is able to tilt up to 90 degrees clockwise in order to better image samples. Caution should be taken to avoid crashing the objective into the sample or stage when head is tilted. \\ {{:tools:keyencemicroscope:lockout.png?400|}}
       - Loosen the tilt lock knob a turn or two max (until the knob loosens)       - Loosen the tilt lock knob a turn or two max (until the knob loosens)
       - With a HAND ON THE MICROSCOPE HEAD rotate the locking lever counter clockwise to its vertical position       - With a HAND ON THE MICROSCOPE HEAD rotate the locking lever counter clockwise to its vertical position
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       - Lock the head in its tilted position using the large tilt lock knob. Make sure knob is snug before letting go of the head but do not overtighten       - Lock the head in its tilted position using the large tilt lock knob. Make sure knob is snug before letting go of the head but do not overtighten
       - If desired angle is greater then 60 degrees, pull the locking pin out away from the microscope before retightening the tilt lock knob. This should allow the microscope head to tilt up to 90 degrees. Be incredibly careful as the stage and objective will become very close at angles above 60 degrees.       - If desired angle is greater then 60 degrees, pull the locking pin out away from the microscope before retightening the tilt lock knob. This should allow the microscope head to tilt up to 90 degrees. Be incredibly careful as the stage and objective will become very close at angles above 60 degrees.
-  - **Changing lighting elements** \\ {{:tools:keyencemicroscope:attatchments.jpg?400|}}+  - **Changing lighting elements** \\ {{:tools:keyencemicroscope:attatchments.png?400|}}
     - Switching to fiberoptic Epi-illumination for 100-1000x objective     - Switching to fiberoptic Epi-illumination for 100-1000x objective
       - With the 100-1000x objective installed turn on the MI-150 fiberoptic lamp       - With the 100-1000x objective installed turn on the MI-150 fiberoptic lamp
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   - **Software lighting and Image Quality**   - **Software lighting and Image Quality**
     - Within Software lighting two tabs are available:     - Within Software lighting two tabs are available:
-      - Brightness \\ {{:tools:keyencemicroscope:lighting.jpg?400|}}+      - Brightness \\ {{:tools:keyencemicroscope:lighting.png?400|}}
         - Adjust shutter speed and camera settings (set to auto)         - Adjust shutter speed and camera settings (set to auto)
-      - Lighting \\ {{:tools:keyencemicroscope:other_lighting.jpg?400|}}+      - Lighting \\ {{:tools:keyencemicroscope:other_lighting.png?400|}}
         - Epi-illumination         - Epi-illumination
           - Adjust/turn on internal objective illumination (press "light" on console to toggle)           - Adjust/turn on internal objective illumination (press "light" on console to toggle)
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       - Select "depth up" from the right hand side bar then "quick composition and 3D"       - Select "depth up" from the right hand side bar then "quick composition and 3D"
       - Focus the microscope slightly below the lowest point on the object \\ {{:tools:keyencemicroscope:focuso_on_bottom.jpg?400|}}       - Focus the microscope slightly below the lowest point on the object \\ {{:tools:keyencemicroscope:focuso_on_bottom.jpg?400|}}
-      - Click "3D Display" on the Keyence control pad and the microscope will scan up the object and create a 3D file which can be analyzed for depth. \\ {{:tools:keyencemicroscope:large_depth_of_field.jpg?400|}}{{:tools:keyencemicroscope:3d_depth_map.jpg?400|}}+      - Click "3D Display" on the Keyence control pad and the microscope will scan up the object and create a 3D file which can be analyzed for depth. \\ {{:tools:keyencemicroscope:large_depth_of_field.jpg?400|}}{{:tools:keyencemicroscope:depth.png?400|}}
   - **Measure**   - **Measure**
     - Measurements can be taken by selecting the "Measure" tab to measure the current view of the microscope or after a Depth up scan by selecting the "measure" tab after scan is complete     - Measurements can be taken by selecting the "Measure" tab to measure the current view of the microscope or after a Depth up scan by selecting the "measure" tab after scan is complete
-      - Select the desired tool and desired points to measure from on the sample. The microscope will automatically display the length of lines or radius of arcs/circles drawn \\ {{:tools:keyencemicroscope:measuring.jpg?400|}}+      - Select the desired tool and desired points to measure from on the sample. The microscope will automatically display the length of lines or radius of arcs/circles drawn \\ {{:tools:keyencemicroscope:measuring.png?400|}}
  
   - **Stitching**   - **Stitching**
-    - Center surround stitching \\ {{:tools:keyencemicroscope:stitch.jpg?400|}}+    - Center surround stitching \\ {{:tools:keyencemicroscope:stitch.png?400|}}
       - Center the sample in the frame and select "stitching" from the sidebar        - Center the sample in the frame and select "stitching" from the sidebar 
       - select "stitch around the current position"       - select "stitch around the current position"
       - click "Start stitching" and press enter to stop the stitching whenever the image collected reaches the desired size.       - click "Start stitching" and press enter to stop the stitching whenever the image collected reaches the desired size.
-    - Stitch after specifying the area \\ {{:tools:keyencemicroscope:stitch_set_bounds.jpg?400|}}+    - Stitch after specifying the area \\ {{:tools:keyencemicroscope:stitch_set_bounds.png?400|}}
       - Click select range       - Click select range
       - Position the microscope as the minimum x position and select left, then maximum x position and select right       - Position the microscope as the minimum x position and select left, then maximum x position and select right
keyence_sop.1605204410.txt.gz · Last modified: 2020/11/12 18:06 by furst