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--- lbf-intro [2024/09/27 21:32] – okorafor
+++ lbf-intro [2024/10/08 21:48] (current) – okorafor
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+=====Introduction=====
-An automated, high-throughput platform for gene assembly, amplification, transformation, strain growth, and metabolite analysis enables the production of bio-based monomers and polymers with precise repeat units, domains and chirality directly from microorganisms. By providing an equipment set focused on automation, control, and high-throughput pathway assembly at the gene level and metabolite detection at the cellular level, the Living Bioreactor will enable biosynthetic manufacturing of commodity monomers and polymers.
+{{:lbf.jpg?nolink&600|}}
-A ThermoFisher Laboratory Automation System (LAS) serves as the cornerstone technology for the facility, enabling execution of automated customized synthetic biology and workflows at >500 samples-per-week. The LAS is equipped with over of over 10 functional instrumental components, including: a state-of-the-art SpinnakerTM microplate robot, automated incubators, reagent dispensers, thermal cyclers, plate sealer, and carousels/racks that are seamlessly integrated through the MOMENTUMTM application programming interface that is fully-compatible with laboratory information management systems (LIMS).
+An automated, high-throughput platform for gene assembly, amplification, transformation, strain growth, and metabolite analysis enables the production of bio-based monomers and polymers with precise repeat units, domains and chirality directly from microorganisms. By providing an equipment set focused on automation, control, and high-throughput pathway assembly at the gene level and metabolite detection at the cellular level, the Living Biofoundry will enable biosynthetic manufacturing of commodity monomers and polymers.
-The capabilities of the Living Biofoundry Facility are supplemented by: (1) a Thermo Fisher TSQ Altis inline triple quadrupole mass spectrometer coupled with an ultra-high-performance liquid chromatograph (UHPLC/MS/MS) for separation and analysis of synthesized bio-derived monomers, (2) Agilent Technologies 7890A gas chromatography system, and (3) BIOFLO CELLIGEN 310 fermenter/bioreactor for scale-up microbial culture.
-===== Fluent Liquid Handler =====
-===== ThermoFisher Laboratory Automation System=====
-===== ThermoFisher TSQ Altis Triple Quadrupole Mass Spectrometer coupled with an Ultra-High-Performance Liquid Chromatograph (UPHLC/MS/MS)====
+|  **Supervisor**        |  **email**  |
+| Michael Lake       |  mlake@cnsi.ucla.edu   |
+| Ikechukwu Okorafor         |  iokorafor@cnsi.ucla.edu   |
-===== SciRobotics Pickolo Colony Picker =====
-===== Fisher Scientific BTX Gemini X2 Electroporation System =====