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microed-sample [2024/10/02 21:18] – elenimicroed-sample [2024/10/02 21:42] (current) – [Sample Preparation] eleni
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 ====== Sample Preparation ====== ====== Sample Preparation ======
 +
 +{{:workflow_diagram_planning_whiteboard_in_green_yellow_modern_professional_style_1_.png?600 |}}
  
 ===== Grids ===== ===== Grids =====
  
  
-| **Grids to Get:**                  Grids 25 um thick, 3 mm in diameter, film on “dull” side, “shiny” side has the metal only, the dull side has the film                          |+|**Thickness:** 25 μm  \\ **Diameter:** 3 mm \\ Film on “dull” side\\ “Shiny” side has the metal only \\                       |
  
   * Ultrathin Carbon Film on a Lacey Carbon Support Film   * Ultrathin Carbon Film on a Lacey Carbon Support Film
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 ===== Instructions for Nanoparticle Dropcast TEM sample preparation ===== ===== Instructions for Nanoparticle Dropcast TEM sample preparation =====
  
-Things you will need:+**Things you will need:**
  
  * Ethanol (or water, IPA, or dimethyl carbonate)\\  * Ethanol (or water, IPA, or dimethyl carbonate)\\
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  * UltraThin 3 nm Carbon Grids\\  * UltraThin 3 nm Carbon Grids\\
  * Glass slide\\  * Glass slide\\
-1. Check the MSDS for mixture with ethanol (make sure the nanoparticles are NOT pyrophoric)\\+**1.** Check the MSDS for mixture with ethanol (make sure the nanoparticles are NOT pyrophoric)\\
  
-2. Use the small metal scooper to extract about ½-1 cms worth of nanoparticles\\+**2.** Use the small metal scooper to extract about ½-1 cms worth of nanoparticles\\
  
-3. Place the nanoparticles in a small vial\\+**3.** Place the nanoparticles in a small vial\\
  
-4. Fill the small vial with ethanol up to the 1 cm below the rim\\+**4.** Fill the small vial with ethanol up to the 1 cm below the rim\\
  
-5. Sonicate the filled small vial for 10 minutes on high power (make sure to find an anti-node)\\+**5.** Sonicate the filled small vial for 10 minutes on high power (make sure to find an anti-node)\\
  
-6. Centrifuge for 20 minute (make sure to balance the centrifuge)\\+**6.** Centrifuge for 20 minute (make sure to balance the centrifuge)\\
  
-7. Use a pipette to collect several uL of fluid just above the concentrated part at the bottom of the vial. See the arrow below . The pipette should just touch above dark part.\\+**7.** Use a pipette to collect several uL of fluid just above the concentrated part at the bottom of the vial. See the arrow below . The pipette should just touch above dark part\\
 {{ :vial2.png?200 |}} {{ :vial2.png?200 |}}
  
-8. Deposit 1 uL of fluid onto the dull (not shiny) side of the grid.\\+**8.** Deposit 1 uL of fluid onto the dull (not shiny) side of the grid\\
  
-9. Let the grid dry on a glass slide.\\+**9.** Let the grid dry on a glass slide\\
  
-10. Vacuum bake at 50-400 degree C for several hours if contamination is found of the surface.\\+**10.** Vacuum bake at 50-400 degree C for several hours if contamination is found of the surface\\
  
-11. Plasma clean as needed.\\+**11.** Plasma clean as needed\\
  
  
  
microed-sample.1727903901.txt.gz · Last modified: by eleni