NSF BioPACIFIC MIP Wiki

====== Analytical/Preparative Hybrid HPLC ======

^                  Shimadzu Nexera HPLC                       ^
|             {{:shimadzunexerahplc.png?400|}}              |                                  
| **Tool Type:**    Analytical/Preparative HPLC                                         |
| **Location:**                   Elings Hall 2411                           |


|  **Supervisor**        |  **Tool Lead**  |     
|  Morgan Bates         |  Zachary Nett  |
|  morganbates@ucsb.edu         |  zjnett@ucsb.edu  |

| **Description:**                Analytical/Preparative Hybrid HPLC                           |
| **Manufacturer:**               Shimadzu Nexera                           |

----

===== About =====

The Shimadzu Nexera Hybrid HPLC system at BioPACIFIC MIP is a unique instrument
designed with dual analytical and preparative 
ow paths. This design allows for the automated
and easy interchange between an analytical tool for method development and fraction purity re-
analysis and a preparative HPLC for fraction collection. This HPLC system also improves upon
the typically UV-based fractionation process of prep chromatography by inclusion of an inline
single quadrupole MS detector (LCMS-2020). Direct on-line mass spectrometry can help ensure
only fractions containing the target compound are collected and potentially eliminate the need for
subsequent offline MS analysis.
----


===== Detailed Specifications =====

**Force Capacity:** 50 kg.f (500N)\\
**Force Resolution:** 0.1 g\\
**Load cells:** 0.5, 5, 30, 50 kg.f \\
**Speed Range:** 0.01 – 40 mm/s\\
**Maximum Aperture:** 370 mm/590mm\\
**Distance Resolution:** 0.001 mm\\
**Data Acquisition Rate:** 2000 pps\\

----

===== Safety Concerns =====

Avoid sudden changes in pressure and temperature 
Avoid any mechanical shock 
Properly dispose of solvents

----
===== Reference Documentation =====

{{ ::hplcmanualshimadzunexera.pdf |}}


-----