labconco_lyophilzer
Differences
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Both sides previous revisionPrevious revisionNext revision | Previous revision | ||
labconco_lyophilzer [2024/10/15 00:28] – mbates | labconco_lyophilzer [2024/10/15 00:31] (current) – mbates | ||
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- | **1. Wake the System:**\\ | + | **1. Wake the System**\\ |
The lyophilizer will start in Sleep mode. Tap the screen to wake it up. | The lyophilizer will start in Sleep mode. Tap the screen to wake it up. | ||
- | **2. Turn on the Collector:**\\ | + | **2. Turn on the Collector**\\ |
On the home screen, select the Collector tab to turn on the collector. The system will begin pumping down to -84°C, which takes approximately 10 minutes. | On the home screen, select the Collector tab to turn on the collector. The system will begin pumping down to -84°C, which takes approximately 10 minutes. | ||
- | **3. Drain the Collector (if needed):**\\ | + | **3. Drain the Collector (if needed)**\\ |
If prompted to "Drain the Collector" | If prompted to "Drain the Collector" | ||
- Insert the waste tube into a solvent waste bottle. | - Insert the waste tube into a solvent waste bottle. | ||
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- Once the solvent has drained, detach the waste line by pressing the detachment button at the port. (Failure to detach the waste line will prevent the system from establishing vacuum.) | - Once the solvent has drained, detach the waste line by pressing the detachment button at the port. (Failure to detach the waste line will prevent the system from establishing vacuum.) | ||
- | **4. Check the Tower and Vacuum Ports:** | + | **4. Check the Tower and Vacuum Ports**\\ |
- | Ensure the tower is seated properly and that all vacuum ports are closed. | + | Ensure the tower is seated properly and that all vacuum ports are closed. |
- | **5. Start the Vacuum** | + | **5. Start the Vacuum**\\ |
- | Go to the Vacuum tab and set the Vacuum Set Point to 0.01 mbar. Click Start. Turn on the secondary pump located below the instrument by pressing the green button. The pump should engage, and the system will begin to pump down. If the vacuum reading stays " | + | Go to the Vacuum tab and set the Vacuum Set Point to 0.01 mbar. Click Start. Turn on the secondary pump located below the instrument by pressing the green button. The pump should engage, and the system will begin to pump down. If the vacuum reading stays " |
- | **6. Attach Your Sample:**\\ | + | **6. Attach Your Sample**\\ |
- | Once your sample is properly frozen, place it into a vacuum jar and attach it to a vacuum port. | + | Once your sample is properly frozen, place it into a vacuum jar and attach it to a vacuum port. Slowly open the vacuum port by turning the black knob. Be cautious: if the vacuum is opened too quickly, or if the sample isn't fully frozen, it may begin to melt. |
- | | + | |
- | **7. Remove the Sample:**\\ | + | **7. Remove the Sample**\\ |
- | After freeze-drying is complete, close the vacuum port and remove your sample. | + | After freeze-drying is complete, close the vacuum port and remove your sample. |
- | **8. Shut Down the System:**\\ | + | **8. Shut Down the System**\\ |
- | Turn off the condenser and vacuum by selecting " | + | Turn off the condenser and vacuum by selecting " |
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labconco_lyophilzer.1728952114.txt.gz · Last modified: 2024/10/15 00:28 by mbates