NSF BioPACIFIC MIP Wiki

====== Zeiss Inverted Microscope ======


^             Zeiss Axio Observer 7                               ^
| {{:zeissinvmicroscope.jpg?400|}} |
| **Tool Type:**                  "Inverted Microscope"                           |
| **Location:**                   "Elings Hall 2411"                           |


|  **Supervisor**        |  **Tool Lead**  |     
|  Juan Manuel Urueña    |  "WW Name"  |
|  jmuruena@ucsb.edu  |  "WW Email"  |

| **Description:**                "Inverted Microscope"|
| **Manufacturer:**               "Zeiss"                           |

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===== About =====

Inverted microscope Axio Observer 7 ACR for transmitted-light brightfield LED, phase contrast, differential interference contrast, fluorescence with Colibri 7; equipped with ACR function, Scanning Stage STEP and Docking station

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===== Detailed Specifications =====
^             Objectives                              ^
{{:microscopeobjectives.jpg?400|}}\\
^             Filter Sets                               ^
{{:fluorescent_cubes.jpg?400|}}\\
^             Camera Sensitivity to different wavelengths    ^
{{:axium720cameraspecs-01.jpg?400|}}

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===== Filter Set Specs =====

Position 1 GFP {{:pos-1-38hegfp.jpg?linkonly|}}\\
Position 2 DsRed {{ :pos-2-43hedsred.jpg?linkonly |}}\\
Position 3 CY5 {{ :pos-3-50cy5.jpg?linkonly |}}\\
Position 4 DAPI FITC TRITC {{ :pos-4-90he_d_g_c3.jpg?linkonly |}}\\
Position 5 96 HE {{ :pos-5-96hebfp.jpg?linkonly |}}

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===== Plate Layout Template =====

96 well plate experiment layout{{ :96_well_plate_layout.pdf |}}
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===== Safety Concerns =====

Read the manufactures manual before first use. If the Zeiss inverted microscope acts in a way that is not described by the manual, turn off the printer and contact Zeiss.\\
	* Never place your finger near the machine until all parts have stopped moving. Moving parts can cause serious injury\\
	* Never clean or service the microscope while it is on\\
	* The microscope uses UV light to excite some dyes. Never look directly at LED light nor expose skin. Serious injury may result from exposure\\
	* Disassembling the microscope may cause an electric shock or damage to the instrument.    Do not disassemble any parts of the microscope not mentioned in the instruction manual.    In case of a problem with the microscope \\

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===== Operating Procedures =====

Well Plate Calibration {{ :multiwellplatecalibration.pdf |}} \\
96 well plate experiment {{ :96-wellexperiment.pdf |}} \\

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===== Reference Documentation =====

{{ :zen_2_blue_edition_-_software_guide.pdf |}}
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===== Training Documentation =====