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====== High-Througput Microindenter ======


^             Optics 11 Pavone                              ^
{{:02_pavone.png?400|}}
| **Tool Type:**                  "High-Trhroughput Microindenter"                           |
| **Location:**                   "Elings Hall 2411"                           |


|  **Supervisor**        |  **Tool Lead**  |     
|  Juan Manuel Urueña    |  "WW Name"  |
|  jmuruena@ucsb.edu  |  "WW Email"  |

| **Description:**                "High-Throughput Microindenter"|
| **Manufacturer:**               "Optics11 Life"                           |

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===== About =====

The Optics11 Pavone is a high-throughput microindenter designed for the mechanical characterization of biological samples. It's a versatile tool capable of measuring the mechanical properties of cells, tissues, and biomaterials at various scales, from subcellular to whole tissues.

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===== Detailed Specifications =====
* **High-Throughput:** Capable of testing up to 2 x 96-well plates in a single run, enabling large-scale experiments.
* **Automated Workflow:** Reduces the need for specialized operators and minimizes hands-on time.
* **Combined Imaging and Indentation:** Offers both bright-field and fluorescence imaging capabilities, allowing for correlation of mechanical properties with morphological features.
* **Environmental Control:** Maintains physiological conditions (temperature, humidity, and gas composition) for long-duration experiments on living samples.
* **Versatile Probe:** A single, pre-calibrated, reusable probe can be used for a wide range of indentation depths and forces.


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===== cantilevers =====
| Stiffness (N/m) | Tip Radius (µm) |
| 0.021 | 25.5 |
| 0.43 | 25 |
| 0.47 | 49.5 |
| 0.43 | 52.5 |
| 3.52 | 48 |
| 53.5 | 25.5 |

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===== Safety Concerns =====

**General Safety Concerns**

* **Electrostatic Discharge (ESD):**
   * Handle components carefully to avoid electrostatic damage.
   * Use ESD mats and grounding straps when working with sensitive electronic components.

* **Laser Safety:**
   * Never look directly into the laser beam.
   * Wear appropriate laser safety goggles.
   * Follow laser safety guidelines and procedures.

* **Mechanical Hazards:**
   * Use caution when handling moving parts.
   * Be aware of potential pinch points and crush hazards.

* **Chemical Hazards:**
   * Handle chemicals with care and wear appropriate protective equipment (gloves, lab coat, safety goggles).
   * Dispose of chemicals properly.
   * Be aware of chemical compatibility and potential hazards.

* **Electrical Hazards:**
   * Avoid working with live electrical circuits.
   * Use proper grounding techniques.
   * Be aware of potential shock hazards.

To avoid breaking the cantilevers, it's crucial to:

* **Handle the probes with care:** Avoid touching the tip.\\
* **Store the probes properly:** Store them in a clean, dust-free environment.\\
* **Calibrate the instrument regularly:** Ensure accurate measurements.\\
* **Follow the manufacturer's guidelines:** Adhere to the recommended procedures for operation and maintenance.\\

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===== Reference Documentation =====

{{ ::pavone_manual_v1_6.2.pdf |}}
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===== Training Documentation =====