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====== Shimadzu Nexera Preparative HPLC-MS ======

^                  Shimadzu Nexera HPLC-MS                       ^
|             {{Prep_hplc.jpg?400|}}             |                                  
| **Tool Type:**    Reverse-phase Preparative Purification                                         |
| **Location:**                   Elings Hall 2411                          |


|  **Principal Scientist**        |  **Training and Operations Lead**  |     
|  Morgan Bates         |  Zachary Nett  |
|  morganbates@ucsb.edu         |  zjnett@ucsb.edu  |

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===== About =====

The Shimadzu Nexera Preparative HPLC system at BioPACIFIC MIP marks a significant advancement in preparative chromatography. Integrating an inline single quadrupole MS detector (LCMS-2020) with the HPLC system, it elevates the standard UV-based fractionation process to allow for direct on-line mass spectrometry. This integration ensures efficient collection of only fractions containing the target compound, significantly reducing or even eliminating the need for subsequent offline MS analysis.

Equipped with a 6-way switch valve, the system enables automated switching between three semi-preparative columns (19 mm ID) and two high-capacity preparative columns (50 mm ID), all installed simultaneously. This feature provides remarkable flexibility in column selection, controlled within the programmed method, and allows for optimized separation and purification processes to meet diverse research requirements.

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===== Reference Documentation =====

{{ :prep_hplc_manual.pdf | NSF BioPACIFIC MIP Preparative HPLC-MS User Manual}}

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