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--- inverted_microscope [2021/07/23 19:34] – created jmuruena
+++ inverted_microscope [2021/08/24 18:42] (current) – [Operating Procedures] jmuruena
@@ Line 2: / Line 2: @@
-^             Mono1 Printer                               ^
+^             Zeiss Axio Observer 7                               ^
 | {{:zeissinvmicroscope.jpg?400|}} |
 | **Tool Type:**                  "Inverted Microscope"                           |
@@ Line 24: / Line 24: @@
 ===== Detailed Specifications =====
-{{:microscopeobjectives.jpg?400|}}
+^             Objectives                              ^
-{{:fluorescent_cubes.jpg?400|}}
+{{:microscopeobjectives.jpg?400|}}\\
+^             Filter Sets                               ^
+{{:fluorescent_cubes.jpg?400|}}\\
+^             Camera Sensitivity to different wavelengths    ^
+{{:axium720cameraspecs-01.jpg?400|}}
+----
+===== Filter Set Specs =====
+Position 1 GFP {{:pos-1-38hegfp.jpg?linkonly|}}\\
+Position 2 DsRed {{ :pos-2-43hedsred.jpg?linkonly |}}\\
+Position 3 CY5 {{ :pos-3-50cy5.jpg?linkonly |}}\\
+Position 4 DAPI FITC TRITC {{ :pos-4-90he_d_g_c3.jpg?linkonly |}}\\
+Position 5 96 HE {{ :pos-5-96hebfp.jpg?linkonly |}}
+----
+===== Plate Layout Template =====
+well plate experiment layout{{ :96_well_plate_layout.pdf |}}
 ----
 ===== Safety Concerns =====
-Read the manufactures manual before first use. If the Mono 1 printer acts in a way that is not described by the manual, turn off the printer and contact monoPRINTER.\\
+Read the manufactures manual before first use. If the Zeiss inverted microscope acts in a way that is not described by the manual, turn off the printer and contact Zeiss.\\
 	* Never place your finger near the machine until all parts have stopped moving. Moving parts can cause serious injury\\
-	* Never clean or service the printer while it is on\\
+	* Never clean or service the microscope while it is on\\
-	* The printer uses UV light for curing. Never look directly at UV light nor expose skin. Serious injury may result from exposure\\
+	* The microscope uses UV light to excite some dyes. Never look directly at LED light nor expose skin. Serious injury may result from exposure\\
-	* Always ensure that equipment is correctly mounted before use. Improperly mounted print beds, build platfomrs can be dangerous. If any equipment appears damaged, turn off the printer, unplug all connections and contact monoPRINTER. \\
+	* Disassembling the microscope may cause an electric shock or damage to the instrument.    Do not disassemble any parts of the microscope not mentioned in the instruction manual.    In case of a problem with the microscope \\
 ----
@@ Line 41: / Line 59: @@
 ===== Operating Procedures =====
-Insert Text Here!
+Well Plate Calibration {{ :multiwellplatecalibration.pdf |}} \\
+well plate experiment {{ :96-wellexperiment.pdf |}} \\
 ----
@@ Line 47: / Line 66: @@
 ===== Reference Documentation =====
-{{ :mono3manual.pdf |}}
+{{ :zen_2_blue_edition_-_software_guide.pdf |}}
 -----
 ===== Training Documentation =====