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--- lbf-las [2025/06/27 18:10] – [Thermo Scientific Multidrop Combi] okorafor
+++ lbf-las [2025/06/28 00:19] (current) – [Automated Thermal Cycler] okorafor
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-The BioTek ELx405 is the first deep well plate washer that aspirates and dispenses into 96- and 384-well deep well and standard height microplates. \\
+**About**
+The BioTek ELx405 is the first deep well plate washer that aspirates and dispenses into 96- and 384-well deep well and standard height microplates.
+It is a versatile, high-performance laboratory instrument designed for automated washing of microplates, supporting a wide range of applications such as ELISA, cell-based assays, immunoassays, etc.
+**Key Features**:
+**Plate Compatibility**: Washes 384-well and 96- well microplates in addition to 24-well and 96-well deep well plates (up to 50 mm in height)
+**Washing Technology**:
+Utilizes a patented Dual-Action™ manifold, enabling independent control of dispense and aspiration for precise washing, overfill washing, and overflow protection.
+Low-flow rates and 20° angled dispensing are available, making it especially suitable for gentle washing of adherent cell-based assays.
+**Fluid Handling**:
+Internal positive displacement pump for accurate fluid delivery, supporting volumes from 50 to 3000 μL/well.
+Optional buffer switching allows for up to four different wash buffers to be used automatically in a single protocol.  \\
 **User Notes** \\
 **Before Use**: Ensure that the waste containers are not full. \\
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 ====Automated Thermal Cycler====
+**About** \\
 The Automated Thermal cycler (ATC) is capable of performing PCRs (polymerase chain reactions) at a high-throughput scale.
+The Thermo Fisher Applied Biosystems Automated Thermal Cycler (ATC) is a compact, robust, and highly integrable PCR instrument designed specifically for automated and high-throughput laboratory workflows. It is engineered to deliver reliable, hands-free PCR amplification, supporting both 96-well and 384-well plate formats, and is optimized for seamless integration with robotic liquid handling systems.
+**Key Features**
+**Flexible Plate and Volume Compatibility**
+Supports 96-well (0.2 mL) and 384-well (0.02 mL) formats.
+**PCR volume range**:
+-well: 10–100 μL for full-skirted plates; 20–100 μL for half-skirted plates
+-well: 5–20 μL for full-skirted plates
+Compatible with ANSI/SBS-standard plates.
+**Performance and Temperature Control**
+Thermal range: 0°C to 105°C, supporting a wide variety of PCR protocols.
+Temperature accuracy: ±0.25°C (35°C to 99.9°C).
+Temperature uniformity: ≤0.5°C (20 seconds after reaching 95°C).
+Maximum block ramp rates: 3.5°C/sec (96-well), 2.8°C/sec (384-well); maximum sample ramp rates: 1.8°C/sec (96-well), 1.6°C/sec (384-well).
+**Automation and Software Integration**
+SiLA Rapid Integration™ standard-compatible software allows straightforward connection to major robotic platforms.
+Prewritten APIs and free desktop software enable both stand-alone operation and full robotic integration, including simulation modes for legacy Applied Biosystems 9700 systems.
 Other features include:
      - Automated lid critical for hands-free operation