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--- microed-sample [2024/10/02 21:14] – eleni
+++ microed-sample [2024/10/02 21:42] (current) – [Sample Preparation] eleni
@@ Line 1: / Line 1: @@
 ====== Sample Preparation ======
+{{:workflow_diagram_planning_whiteboard_in_green_yellow_modern_professional_style_1_.png?600 |}}
 ===== Grids =====
-| **Grids to Get:**                  Grids 25 um thick, 3 mm in diameter, film on “dull” side, “shiny” side has the metal only, the dull side has the film                          |
+|**Thickness:** 25 μm  \\ **Diameter:** 3 mm \\ Film on “dull” side\\ “Shiny” side has the metal only \\                       |
   * Ultrathin Carbon Film on a Lacey Carbon Support Film
@@ Line 25: / Line 27: @@
 ===== Instructions for Nanoparticle Dropcast TEM sample preparation =====
-Things you will need:
+**Things you will need:**
 	* Ethanol (or water, IPA, or dimethyl carbonate)\\
@@ Line 35: / Line 37: @@
 	* UltraThin 3 nm Carbon Grids\\
 	* Glass slide\\
-. Check the MSDS for mixture with ethanol (make sure the nanoparticles are NOT pyrophoric)\\
+**1.** Check the MSDS for mixture with ethanol (make sure the nanoparticles are NOT pyrophoric)\\
-. Use the small metal scooper to extract about ½-1 cms worth of nanoparticles\\
+**2.** Use the small metal scooper to extract about ½-1 cms worth of nanoparticles\\
-. Place the nanoparticles in a small vial\\
+**3.** Place the nanoparticles in a small vial\\
-. Fill the small vial with ethanol up to the 1 cm below the rim\\
+**4.** Fill the small vial with ethanol up to the 1 cm below the rim\\
-. Sonicate the filled small vial for 10 minutes on high power (make sure to find an anti-node)\\
+**5.** Sonicate the filled small vial for 10 minutes on high power (make sure to find an anti-node)\\
-. Centrifuge for 20 minute (make sure to balance the centrifuge)\\
+**6.** Centrifuge for 20 minute (make sure to balance the centrifuge)\\
-. Use a pipette to collect several uL of fluid just above the concentrated part at the bottom of the vile. See the arrow below . The pipette should just touch above dark part.\\
+**7.** Use a pipette to collect several uL of fluid just above the concentrated part at the bottom of the vial. See the arrow below . The pipette should just touch above dark part\\
 {{ :vial2.png?200 |}}
-Read the manufactures manual before first use. If the Lumen X acts in a way that is not described by the manual, turn off the printer and contact CELLINK.\\
+**8.** Deposit 1 uL of fluid onto the dull (not shiny) side of the grid\\
-* Never reach into the instrument when parts are moving\\
-	* Always wear protective goggles, gloves, and lab coat while handling hazardous materials\\
+**9.** Let the grid dry on a glass slide\\
-	* Always ensure that the light shield covers the print area when the printer is in operation. Wear protective safety goggles when using the printer. Do not look directly at the light projected during printing\\
-	* The printer has heated surfaces that can reach temperature up to 50 degrees Celsius. Never touch these surfaces when using the heating function. Allow things to cool before opening, touching the printer \\
+**10.** Vacuum bake at 50-400 degree C for several hours if contamination is found of the surface\\
-	* Always ensure that equipment is correctly mounted before use. Improperly mounted print beds and cables can be dangerous. If any equipment appears damaged, turn off the printer, unplug all connections and contact CELLINK. \\
-	* Make sure the build platform is not secure when preparing it for your print. As the build platform is lowered to your substrate damage might occur if the build platform is rigidity fixed to the stage.
+**11.** Plasma clean as needed\\
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