• Ultrathin Carbon Film on a Lacey Carbon Support Film
• https://www.tedpella.com/Support_Films_html/Support_Films_and_Substrates_Overview.aspx
• 01824 Ultrathin Carbon Film on Lacey Carbon Support Film, 400 mesh, Copper
• 160 PELCO®️ TEM Grid Storage Box

Put a small amount of powder in Eppendorf tub (enough to coat the bottom). Put one grid into the tube. Close the tube and shake for 1 minute. Open the tube and remove the grid. The grid should have enough material on it and is ready to be imaged in the TEM.

Things you will need:

• Ethanol (or water, IPA, or dimethyl carbonate)
  
• A small metal scooper
  
• A sonicator
  
• Several small vials that are centrifuge compatible
  
• A centrifuge
  
• Nanoparticles
  
• UltraThin 3 nm Carbon Grids
  
• Glass slide
  

1. Check the MSDS for mixture with ethanol (make sure the nanoparticles are NOT pyrophoric)

2. Use the small metal scooper to extract about ½-1 cms worth of nanoparticles

3. Place the nanoparticles in a small vial

4. Fill the small vial with ethanol up to the 1 cm below the rim

5. Sonicate the filled small vial for 10 minutes on high power (make sure to find an anti-node)

6. Centrifuge for 20 minute (make sure to balance the centrifuge)

7. Use a pipette to collect several uL of fluid just above the concentrated part at the bottom of the vile. See the arrow below . The pipette should just touch above dark part.

Read the manufactures manual before first use. If the Lumen X acts in a way that is not described by the manual, turn off the printer and contact CELLINK.
* Never reach into the instrument when parts are moving

• Always wear protective goggles, gloves, and lab coat while handling hazardous materials
  
• Always ensure that the light shield covers the print area when the printer is in operation. Wear protective safety goggles when using the printer. Do not look directly at the light projected during printing
  
• The printer has heated surfaces that can reach temperature up to 50 degrees Celsius. Never touch these surfaces when using the heating function. Allow things to cool before opening, touching the printer
  
• Always ensure that equipment is correctly mounted before use. Improperly mounted print beds and cables can be dangerous. If any equipment appears damaged, turn off the printer, unplug all connections and contact CELLINK.
  
• Make sure the build platform is not secure when preparing it for your print. As the build platform is lowered to your substrate damage might occur if the build platform is rigidity fixed to the stage.

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