Table of Contents
High-Througput Microindenter
| Optics 11 Pavone |
|---|
| Tool Type: “High-Trhroughput Microindenter” |
| Location: “Elings Hall 2411” |
| Supervisor | Tool Lead |
| Juan Manuel Urueña | “WW Name” |
| jmuruena@ucsb.edu | “WW Email” |
| Description: “High-Throughput Microindenter” |
| Manufacturer: “Optics11 Life” |
About
The Optics11 Pavone is a high-throughput microindenter designed for the mechanical characterization of biological samples. It's a versatile tool capable of measuring the mechanical properties of cells, tissues, and biomaterials at various scales, from subcellular to whole tissues.
Detailed Specifications
* High-Throughput: Capable of testing up to 2 x 96-well plates in a single run, enabling large-scale experiments. * Automated Workflow: Reduces the need for specialized operators and minimizes hands-on time. * Combined Imaging and Indentation: Offers both bright-field and fluorescence imaging capabilities, allowing for correlation of mechanical properties with morphological features. * Environmental Control: Maintains physiological conditions (temperature, humidity, and gas composition) for long-duration experiments on living samples. * Versatile Probe: A single, pre-calibrated, reusable probe can be used for a wide range of indentation depths and forces.
cantilevers
| Stiffness (N/m) | Tip Radius (µm) |
| 0.021 | 25.5 |
| 0.43 | 25 |
| 0.47 | 49.5 |
| 0.43 | 52.5 |
| 3.52 | 48 |
| 53.5 | 25.5 |
Safety Concerns
General Safety Concerns
* Electrostatic Discharge (ESD):
- Handle components carefully to avoid electrostatic damage.
- Use ESD mats and grounding straps when working with sensitive electronic components.
* Laser Safety:
- Never look directly into the laser beam.
- Wear appropriate laser safety goggles.
- Follow laser safety guidelines and procedures.
* Mechanical Hazards:
- Use caution when handling moving parts.
- Be aware of potential pinch points and crush hazards.
* Chemical Hazards:
- Handle chemicals with care and wear appropriate protective equipment (gloves, lab coat, safety goggles).
- Dispose of chemicals properly.
- Be aware of chemical compatibility and potential hazards.
* Electrical Hazards:
- Avoid working with live electrical circuits.
- Use proper grounding techniques.
- Be aware of potential shock hazards.
To avoid breaking the cantilevers, it's crucial to:
* Handle the probes with care: Avoid touching the tip.
* Store the probes properly: Store them in a clean, dust-free environment.
* Calibrate the instrument regularly: Ensure accurate measurements.
* Follow the manufacturer's guidelines: Adhere to the recommended procedures for operation and maintenance.