Table of Contents
Zeiss Inverted Microscope
| Zeiss Axio Observer 7 |
|---|
 |
| Tool Type: “Inverted Microscope” |
| Location: “Elings Hall 2411” |
| Supervisor | Tool Lead |
| Juan Manuel Urueña | “WW Name” |
| jmuruena@ucsb.edu | “WW Email” |
| Description: “Inverted Microscope” |
| Manufacturer: “Zeiss” |
About
Inverted microscope Axio Observer 7 ACR for transmitted-light brightfield LED, phase contrast, differential interference contrast, fluorescence with Colibri 7; equipped with ACR function, Scanning Stage STEP and Docking station
Detailed Specifications
| Objectives |
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| Filter Sets |
|---|
| Camera Sensitivity to different wavelengths |
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Filter Set Specs
Position 1 GFP pos-1-38hegfp.jpg
Position 2 DsRed pos-2-43hedsred.jpg
Position 3 CY5 pos-3-50cy5.jpg
Position 4 DAPI FITC TRITC pos-4-90he_d_g_c3.jpg
Position 5 96 HE pos-5-96hebfp.jpg
Plate Layout Template
96 well plate experiment layout96_well_plate_layout.pdf
Safety Concerns
Read the manufactures manual before first use. If the Zeiss inverted microscope acts in a way that is not described by the manual, turn off the printer and contact Zeiss.
- Never place your finger near the machine until all parts have stopped moving. Moving parts can cause serious injury
- Never clean or service the microscope while it is on
- The microscope uses UV light to excite some dyes. Never look directly at LED light nor expose skin. Serious injury may result from exposure
- Disassembling the microscope may cause an electric shock or damage to the instrument. Do not disassemble any parts of the microscope not mentioned in the instruction manual. In case of a problem with the microscope
Operating Procedures
Well Plate Calibration multiwellplatecalibration.pdf
96 well plate experiment 96-wellexperiment.pdf